The Test match in the 2025 Women's Ashes in Australia will be a day-night game at the 100,000-capacity Melbourne Cricket Ground.
The Test returns to a four-day match after it was extended to five days during the 2023 series in England.
The pink-ball game, which starts on 30 January, will also end the multi-format series for the first time since it was introduced in 2015.
Australia retained the Ashes with an 8-8 draw last year in England.
"[The Test is] an amazing opportunity for the team to play at such an iconic Australian cricket venue and in such a big occasion like an Ashes series," said Australia all-rounder Ellyse Perry.
"That's the next evolution for women's cricket [and] more globally as well for women's sport - we're starting to see that happen more and more often across the board."
Perry added she would "skew towards" favouring a five-day Test because of the number of four-day games that end in a draw, but "this is going to be a question that keeps popping up".
The multi-format Ashes will also consist of three one-day internationals and three T20s - each worth two points. The Test is worth four points.
The ODIs will start the series from 12 January, before the T20s begin on 20 January.
Australia won the Test in 2023 but England claimed both white-ball series 2-1.
England last won the Women's Ashes in 2014, with a 10-8 victory down under.
2025 Women's Ashes schedule
ODI series
12 January: North Sydney Oval, Sydney
14 January: Junction Oval, Melbourne
17 January: Bellerive Oval, Hobart
T20 series
20 January: Sydney Cricket Ground, Sydney
23 January: Manuka Oval, Canberra
25 January: Adelaide Oval, Adelaide
Test match
30 January-2 February: MCG, Melbourne
Source: BBC
BDST: 1019 HRS, MAR 27, 2024
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